Antiproliferative effect of desferrioxamine on vascular smooth muscle cells in vitro and in vivo.
نویسندگان
چکیده
Vascular smooth muscle cell (VSMC) growth is a primary component of accelerated and spontaneous atherosclerosis. Previous studies have shown that iron may be involved in the control of enzymatic activities that modulate DNA synthesis in human cells. In this study the effects of the iron chelator desferrioxamine on in vitro and in vivo VSMC proliferation were tested. Rat VSMCs in culture and a rabbit model of carotid artery balloon injury were used. Desferrioxamine showed a significant inhibitory effect on [3H]thymidine incorporation in cell cultures that was antagonized by iron supplementation. Desferrioxamine also provided effective preventive myointimal VSMC proliferation as assessed by bromodeoxyuridine labeling and morphometric analysis of endoluminal stenosis. These experiments suggested that iron may be involved in the control of VSMC proliferation and that desferrioxamine may have a role in preventing VSMC growth and myointimal proliferative lesions.
منابع مشابه
The effect of adrenomedullin and proadrenomedullin N- terminal 20 peptide on angiotensin II induced vascular smooth muscle cell proliferation
Objective(s): The study aimed to investigate the effects of adrenomedullin (ADM) and proadrenomedullin N- terminal 20 peptide (PAMP) on angiotensin II (AngII)-stimulated proliferation in vascular smooth muscle cells (VSMCs). Materials and Methods: Thoracic aorta was obtained from Wistar rats and VSMCs were isolated from aorta tissues and then cultured. In vitro cultured VSMCs were stimulated w...
متن کاملAntinociceptive and Smooth Muscle Relaxant Activity ofCroton tiglium L Seed: An In-vitro and In-vivo Study
The seed of Croton tiglium L. (SCT) is a well known folk medicine. In China, it has used to treat gastrointestinal disorders, intestinal inflammation, rheumatism, and so on. Previous studies established its purgative and inflammation properties. In addition, the effects of essential oil of SCT on intestinal transit and gastrointestinal tract has been studied. In the present study, we evalu...
متن کاملAntinociceptive and Smooth Muscle Relaxant Activity ofCroton tiglium L Seed: An In-vitro and In-vivo Study
The seed of Croton tiglium L. (SCT) is a well known folk medicine. In China, it has used to treat gastrointestinal disorders, intestinal inflammation, rheumatism, and so on. Previous studies established its purgative and inflammation properties. In addition, the effects of essential oil of SCT on intestinal transit and gastrointestinal tract has been studied. In the present study, we evalu...
متن کاملEffect of Oxidized Low Density Lipoprotein on the Expression of Runx2 and SPARC Genes in Vascular Smooth Muscle Cells
Background: Vascular calcification is an important stage in atherosclerosis. During this stage, vascular smooth muscle cells (VSMC) synthesize many osteogenic factors such as osteonectin (encoded by SPARC). Oxidative stress plays a critical role in atherosclerosis progression, and its accumulation in the vascular wall stimulates the development of atherosclerosis and vascular calcification. The...
متن کاملStructural determinants of the capacity of heparin to inhibit the proliferation of vascular smooth muscle cells. II. Evidence for a pentasaccharide sequence that contains a 3-O-sulfate group
Previous work from our laboratory has demonstrated that both anticoagulant and nonanticoagulant heparin species can inhibit the proliferation of vascular smooth muscle cells in vivo and in vitro. In this communication, we report studies on the structure-function relationships of heparin to its antiproliferative effect on vascular smooth muscle cells. These structure-function studies were carrie...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Arteriosclerosis and thrombosis : a journal of vascular biology
دوره 14 2 شماره
صفحات -
تاریخ انتشار 1994